9K2F
Crystal Structure of CyaF/SAH in open conformational state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2024-01-11 |
| Detector | RIGAKU |
| Wavelength(s) | 1.54184 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 40.355, 103.045, 122.381 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.020 - 2.700 |
| R-factor | 0.22456 |
| Rwork | 0.222 |
| R-free | 0.27794 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.480 |
| Data reduction software | CrysalisPro |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.020 | 2.830 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Number of reflections | 14619 | 1887 |
| <I/σ(I)> | 9.7 | |
| Completeness [%] | 99.7 | |
| Redundancy | 5.7 | |
| CC(1/2) | 0.995 | 0.810 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 8.5 | 289 | 30% w/v PEG 2000,0.12 M Ethylene Glycols, 0.1 M Bicine pH 8.5 |
