9K22
A novel histone H3K27 reader, CBFA2T2, inhibits H3K27me3 demethylation and tumor growth by regulating metabolic genes and metabolite levels
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-07-21 |
| Detector | DECTRIS PILATUS3 X 6M |
| Wavelength(s) | 0.97861 |
| Spacegroup name | P 42 21 2 |
| Unit cell lengths | 51.744, 51.744, 120.178 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.870 - 2.810 |
| R-factor | 0.2511 |
| Rwork | 0.249 |
| R-free | 0.29910 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.162 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1-4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.860 |
| High resolution limit [Å] | 2.810 | 2.810 |
| Rmeas | 0.124 | 0.687 |
| Rpim | 0.026 | 0.139 |
| Number of reflections | 4389 | 4420 |
| <I/σ(I)> | 5.2 | |
| Completeness [%] | 97.9 | |
| Redundancy | 13.3 | |
| CC(1/2) | 0.993 | 0.973 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 28%v/v 2-Propanol, 0.1M Bis-Tris pH 6.5, and 3%v/v polyethylene glycol 200 |
