9K1U
crystal structure of Rtks and Ppka complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-01-07 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97852 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 38.552, 107.459, 54.322 |
| Unit cell angles | 90.00, 107.91, 90.00 |
Refinement procedure
| Resolution | 37.250 - 1.792 |
| R-factor | 0.2008 |
| Rwork | 0.199 |
| R-free | 0.24280 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 9k0l |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.109 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.19.2_4158: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.830 |
| High resolution limit [Å] | 1.792 | 1.800 |
| Rpim | 0.036 | 0.620 |
| Number of reflections | 39167 | 1941 |
| <I/σ(I)> | 34.6 | 1 |
| Completeness [%] | 99.4 | 97.9 |
| Redundancy | 5.5 | 4.4 |
| CC(1/2) | 0.996 | 0.514 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 291 | PEG 3350 |
