9K1P

crystal structure of Rtks- C2221

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-11-09
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.919
Spacegroup name	C 2 2 21
Unit cell lengths	153.721, 228.332, 151.623
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.060 - 2.890
R-factor	0.2276
Rwork	0.226
R-free	0.27230
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	9k0l
RMSD bond length	0.012
RMSD bond angle	1.741
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((1.19.2_4158: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.950
High resolution limit [Å]	2.890	2.900
Rpim	0.064	0.341
Number of reflections	59071	2614
<I/σ(I)>	12	1
Completeness [%]	98.8	89.1
Redundancy	13	11.3
CC(1/2)	0.806	0.751

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	PEG 3350