Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-06-25 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 72.884, 54.235, 87.853 |
| Unit cell angles | 90.00, 107.82, 90.00 |
Refinement procedure
| Resolution | 41.820 - 2.090 |
| R-factor | 0.1977 |
| Rwork | 0.195 |
| R-free | 0.23920 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.173 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.18.1_3865: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.140 |
| High resolution limit [Å] | 2.090 | 2.100 |
| Rmerge | 0.109 | 0.809 |
| Rpim | 0.059 | 0.399 |
| Number of reflections | 38278 | 1907 |
| <I/σ(I)> | 13.3 | 2.2 |
| Completeness [%] | 98.8 | 99.7 |
| Redundancy | 6.4 | 5.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 291 | 0.2 M Sodium formate, 16 % w/v Polyethylene glycol 3350, 0.1 M MES pH 6.5 |
