9K1G
COMPLEX STRUCTURE OF ENDO-1,3-FUCANASE (FUN174Sb) FROM GH174 FAMILY WITH FUCOTETRAOSE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-06-28 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97923 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 62.635, 92.051, 75.806 |
| Unit cell angles | 90.00, 104.53, 90.00 |
Refinement procedure
| Resolution | 36.660 - 2.500 |
| R-factor | 0.2479 |
| Rwork | 0.245 |
| R-free | 0.28600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.618 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.660 | 2.600 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.177 | 0.676 |
| Rmeas | 0.193 | 0.733 |
| Rpim | 0.076 | 0.282 |
| Total number of observations | 184830 | 21534 |
| Number of reflections | 28897 | 3251 |
| <I/σ(I)> | 9.1 | 3 |
| Completeness [%] | 99.9 | |
| Redundancy | 6.4 | 6.6 |
| CC(1/2) | 0.993 | 0.896 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 290 | 0.15 M Potassium bromide, 30% w/v Polyethylene glycol monomethyl ether 2,000 |
