9JZT

Crystal structure of ZBTB20 in complex with mouse AFP promoter

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-10-31
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97923
Spacegroup name	P 42 21 2
Unit cell lengths	103.944, 103.944, 77.216
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	53.240 - 3.100
R-factor	0.2065
Rwork	0.205
R-free	0.24890
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	AlphaFold
RMSD bond length	0.003
RMSD bond angle	0.507
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	61.980	3.310
High resolution limit [Å]	3.100	3.100
Rmerge	0.320	1.940
Rmeas	0.330	1.997
Rpim	0.078	0.467
Total number of observations		24682
Number of reflections	8098	1415
<I/σ(I)>	9.7	1.9
Completeness [%]	99.7
Redundancy	16.7	17.4
CC(1/2)	0.997	0.980

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.12 M Ethylene glycols, 0.1 M Tris (base), Bicine pH 8.5, 18% Ethylene glycol, PEG 4K