9JYW
Crystal structure of the gamma-carbonic anhydrase from the polyextremophilic bacterium Aeribacillus pallidus
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 5C (4A) |
| Synchrotron site | PAL/PLS |
| Beamline | 5C (4A) |
| Temperature [K] | 77 |
| Detector technology | PIXEL |
| Collection date | 2020-10-28 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 118.911, 110.672, 98.578 |
| Unit cell angles | 90.00, 118.80, 90.00 |
Refinement procedure
| Resolution | 40.360 - 1.700 |
| R-factor | 0.21475 |
| Rwork | 0.213 |
| R-free | 0.24531 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6ive |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.566 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.360 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rpim | 0.036 | 0.189 |
| Number of reflections | 113523 | 11635 |
| <I/σ(I)> | 14.9 | 47.4 |
| Completeness [%] | 97.6 | |
| Redundancy | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 295 | 0.1 M Tris pH 7.5, 14% (w/v) polyethylene glycol 400, and 200 mM ZnSO4 |
