9JXC
Crystal Structure of AbOhr-R15C
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-06-25 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 73.129, 51.348, 82.047 |
| Unit cell angles | 90.00, 103.57, 90.00 |
Refinement procedure
| Resolution | 43.210 - 2.100 |
| R-factor | 0.20615 |
| Rwork | 0.203 |
| R-free | 0.25746 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.607 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.880 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.085 | 0.239 |
| Rpim | 0.035 | 0.096 |
| Number of reflections | 33504 | 3328 |
| <I/σ(I)> | 14.8 | 6.2 |
| Completeness [%] | 96.0 | 96.9 |
| Redundancy | 6.8 | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.2 M AMMONIUM SULFATE, 18 % w/v Polyethylene glycol 3350, 0.1 M MES pH 6.0 |
