9JWR

Crystal structure of Oryza sativa fibrillin 5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-10-04
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.979183
Spacegroup name	P 1 21 1
Unit cell lengths	40.586, 83.232, 90.035
Unit cell angles	90.00, 94.05, 90.00

Refinement procedure

Resolution	31.180 - 1.700
R-factor	0.1964
Rwork	0.195
R-free	0.23030
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.046
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	31.180	1.761
High resolution limit [Å]	1.700	1.700
Number of reflections	65901	6518
<I/σ(I)>	10.99
Completeness [%]	99.9
Redundancy	10.063
CC(1/2)	0.997	0.835

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	Tacsimate, MES, PEG4000