9JWH
Crystal structure of native bovine pancreatic RNAs A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | BRUKER D8 VENTURE |
| Temperature [K] | 100 |
| Detector technology | CMOS |
| Collection date | 2022-10-13 |
| Detector | BRUKER PHOTON 100 |
| Wavelength(s) | 1.5406 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 64.351, 64.351, 63.817 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 17.840 - 1.850 |
| R-factor | 0.18441 |
| Rwork | 0.182 |
| R-free | 0.22301 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1eic |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.779 |
| Data reduction software | PROTEUM PLUS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 17.840 | 1.850 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.769 | |
| Number of reflections | 13430 | 827 |
| <I/σ(I)> | 20 | 1.9 |
| Completeness [%] | 99.9 | |
| Redundancy | 3.8 | |
| CC(1/2) | 0.990 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 292 | 24-34% (NH4)2SO4, 0.1M sodium acetate buffer, super saturated NaCl |
