9JVU
Crystal structure of F10 core protein from Monkeypox virus reveals its potential inhibitors
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL02U1 |
| Synchrotron site | SSRF |
| Beamline | BL02U1 |
| Temperature [K] | 289.15 |
| Detector technology | PIXEL |
| Collection date | 2022-11-26 |
| Detector | DECTRIS EIGER2 S 9M |
| Wavelength(s) | 0.983 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 62.834, 69.312, 64.829 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.660 - 1.510 |
| R-factor | 0.1889 |
| Rwork | 0.187 |
| R-free | 0.22120 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6rfg |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.947 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.21.2_5419: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.560 | 1.590 |
| High resolution limit [Å] | 1.510 | 1.510 |
| Rmerge | 0.088 | 0.770 |
| Number of reflections | 22447 | 3210 |
| <I/σ(I)> | 9.2 | |
| Completeness [%] | 99.9 | |
| Redundancy | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289.15 | 0.2 M ammonium sulfate, 0.1 M Bis-Tris, pH 5.5, and 25% w/v polyethylene glycol 3350 |
