9JVT

Structure of the C-terminal 4 domains (V4-V6-HP) villin bound to an actin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-07-20
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1
Spacegroup name	C 1 2 1
Unit cell lengths	131.122, 97.689, 134.308
Unit cell angles	90.00, 103.95, 90.00

Refinement procedure

Resolution	46.620 - 3.290
R-factor	0.2433
Rwork	0.241
R-free	0.26820
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	9jus
RMSD bond length	0.002
RMSD bond angle	0.588
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.17.1_3660: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.620	3.520
High resolution limit [Å]	3.290	3.290
Rmerge	0.124	0.991
Rmeas	0.146	1.163
Rpim	0.077	0.605
Number of reflections	24970	2432
<I/σ(I)>	7.6	1.3
Completeness [%]	99.5
Redundancy	3.5
CC(1/2)		0.570

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1 M Tris, pH 8.0 50% PEG 400 0.2 M Li2SO4 1 mM CaCl2