9JVR

De novo designed GFP 1GFL-15

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-06-20
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	P 1
Unit cell lengths	51.698, 65.798, 88.491
Unit cell angles	70.31, 89.84, 74.36

Refinement procedure

Resolution	27.640 - 2.610
R-factor	0.2493
Rwork	0.247
R-free	0.29990
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.860
Data reduction software	autoPROC (1.0.5)
Data scaling software	Aimless (0.7.7)
Phasing software	PHASER (2.8.3)
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	83.080	2.180
High resolution limit [Å]	2.070	2.070
Rmerge	0.057	0.648
Rmeas	0.069	0.916
Rpim	0.038	0.648
Number of reflections	31485	2277
<I/σ(I)>	11.9	0.9
Completeness [%]	71.8
Redundancy	2.9
CC(1/2)	0.990	0.495

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	200mM NH4Cl, 20%PEG3350