9JVB
Trypanosoma brucei strain TREU927 TFIIS2-1 LW domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL02U1 |
| Synchrotron site | SSRF |
| Beamline | BL02U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-12-30 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.978975 |
| Spacegroup name | P 1 |
| Unit cell lengths | 60.510, 80.937, 86.793 |
| Unit cell angles | 116.78, 96.47, 105.93 |
Refinement procedure
| Resolution | 32.820 - 3.500 |
| R-factor | 0.2775 |
| Rwork | 0.276 |
| R-free | 0.31200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.872 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (dev_5463) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.625 |
| High resolution limit [Å] | 3.500 | 3.500 |
| Rmerge | 0.069 | 0.743 |
| Rmeas | 0.082 | 0.866 |
| Rpim | 0.043 | 0.444 |
| Number of reflections | 16881 | 1718 |
| <I/σ(I)> | 11.43 | 1.71 |
| Completeness [%] | 97.5 | |
| Redundancy | 3.6 | |
| CC(1/2) | 0.999 | 0.697 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 289 | 2.4 M Ammonium phosphate dibasic 0.1 M Tris pH 8.5 |
