9JVA

Three-dimensional structure of homo-dimer of cystathione beta lyase/PLP complex from lactobacillus delbrueckii(LdPatB)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	CCD
Collection date	2024-03-25
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97
Spacegroup name	C 1 2 1
Unit cell lengths	115.661, 94.218, 84.762
Unit cell angles	90.00, 126.97, 90.00

Refinement procedure

Resolution	33.860 - 2.080
Rwork	0.166
R-free	0.19450
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (1.16-3549-000)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	66.000	1.640
High resolution limit [Å]	1.560	1.560
Rmerge	0.099	0.534
Number of reflections	65530	1348
<I/σ(I)>	10
Completeness [%]	99.6
Redundancy	5.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291.15	0.2 M Magnesium formate dihydrate (15-25 % w/v) Polyethylene glycol 3350