9JUR

Crystal Structure of NHL domain of human E3 ubiquitin-protein ligase TRIM71

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-04-02
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97918
Spacegroup name	P 1 21 1
Unit cell lengths	45.674, 64.544, 46.139
Unit cell angles	90.00, 110.97, 90.00

Refinement procedure

Resolution	32.630 - 2.850
R-factor	0.2088
Rwork	0.207
R-free	0.25420
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.500
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.19_4092: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	32.633	3.000
High resolution limit [Å]	2.850	2.850
Rmerge	0.124	0.245
Number of reflections	5652	250
<I/σ(I)>	7.7	3.9
Completeness [%]	96.0
Redundancy	2.8	2.8
CC(1/2)	0.954	0.894

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	30% v/v Polyethylene glycol monomethyl ether 550, 0.05 M Magnesium chloride hexahydrate, 0.1 M HEPES pH 7.5