9JTQ
Crystal structure of the light-driven proton pump heimdallarchaeial rhodopsin HeimdallR1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL32XU |
| Synchrotron site | SPring-8 |
| Beamline | BL32XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-06-20 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 51.590, 60.370, 86.100 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.220 - 2.000 |
| R-factor | 0.2028 |
| Rwork | 0.201 |
| R-free | 0.23380 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.585 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.19_4092: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.220 | 2.071 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 1.054 | |
| Rmeas | 1.069 | |
| Rpim | 0.177 | |
| Number of reflections | 18782 | 1831 |
| <I/σ(I)> | 13.5 | 1.33 |
| Completeness [%] | 99.9 | 99.95 |
| Redundancy | 35.8 | 35 |
| CC(1/2) | 0.997 | 0.525 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 3.9 | 293 | PEG 300, sodium acetate, potassium thiocyanate |
