9JTP
Crystal Structure of chitinase (E167Q) from the carnivorous plant Drosera adelae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-11-15 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 43.120, 63.247, 72.894 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.770 - 1.570 |
| R-factor | 0.20358 |
| Rwork | 0.202 |
| R-free | 0.23438 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.597 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.770 | 1.600 |
| High resolution limit [Å] | 1.570 | 1.570 |
| Rmerge | 0.029 | 0.404 |
| Rmeas | 0.032 | 0.472 |
| Rpim | 0.013 | 0.237 |
| Number of reflections | 26673 | 833 |
| <I/σ(I)> | 33.7 | 2.7 |
| Completeness [%] | 93.7 | 60.4 |
| Redundancy | 5.9 | 3.5 |
| CC(1/2) | 0.873 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 15% PEG 8000 40% 2-Propanol 0.1 M Imidazole pH 6.5 |
