9JSA
CatPSAM-H8
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-12-18 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 36.205, 79.450, 227.260 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.200 |
| R-factor | 0.2645 |
| Rwork | 0.263 |
| R-free | 0.28830 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.878 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.171) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.210 | 2.120 |
| High resolution limit [Å] | 2.060 | 2.060 |
| Rmerge | 0.114 | 1.004 |
| Number of reflections | 41886 | 33997 |
| <I/σ(I)> | 14 | 2.3 |
| Completeness [%] | 100.0 | |
| Redundancy | 9.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1M HEPES pH7.5, 25% v/v PEG Smear Medium, protein 10.14 mg/mL |
