9JRY
Crystal structure of FiDCB, a dual-cysteine cyanobacterial phytochrome of Fischerella sp. PCC 9605
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 11C |
| Synchrotron site | PAL/PLS |
| Beamline | 11C |
| Temperature [K] | 110 |
| Detector technology | PIXEL |
| Collection date | 2024-03-22 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 104.551, 147.131, 88.076 |
| Unit cell angles | 90.00, 99.14, 90.00 |
Refinement procedure
| Resolution | 37.430 - 3.350 |
| R-factor | 0.2816 |
| Rwork | 0.280 |
| R-free | 0.29680 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6bap |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.636 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.21.1_5286) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.430 | 3.450 |
| High resolution limit [Å] | 3.349 | 3.350 |
| Number of reflections | 17035 | 867 |
| <I/σ(I)> | 34.28 | |
| Completeness [%] | 89.9 | |
| Redundancy | 4.5 | |
| CC(1/2) | 1.000 | 1.000 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | 0.1 M BICINE (pH 8.0), 181.8 mM NaCl, 9% 2-propanol, 23~34.5% PEG1500 |
