9JRV

Pilin PilA from Burkholderia cenocepacia

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2023-04-08
Detector	RAYONIX MX-300
Wavelength(s)	0.97872
Spacegroup name	P 21 21 21
Unit cell lengths	31.190, 62.160, 138.219
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.430 - 1.500
R-factor	0.1508
Rwork	0.148
R-free	0.19560
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.841
Data reduction software	MOSFLM (7.4.0)
Data scaling software	Aimless (0.7.9)
Phasing software	PHASER (2.8.3)
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.430	1.530
High resolution limit [Å]	1.500	1.500
Rmerge	0.099	1.185
Rmeas	0.103	1.248
Rpim	0.027	0.388
Number of reflections	44186	2118
<I/σ(I)>	15.1	2
Completeness [%]	100.0	100
Redundancy	14.1	10.1
CC(1/2)	0.999	0.749

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	298	100 mM sodium cacodylate, 200 mM sodium acetate, 30% PEG 8,000