9JRC

Crystal structure of SARS-CoV-2 receptor-binding domain complexed with squirrel ACE2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-07-20
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.98
Spacegroup name	I 41 2 2
Unit cell lengths	194.135, 194.135, 148.162
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.650 - 3.160
R-factor	0.1985
Rwork	0.197
R-free	0.22850
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6m0j
RMSD bond length	0.011
RMSD bond angle	1.289
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.19.1_4122: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.270
High resolution limit [Å]	3.160	3.160
Number of reflections	24223	2284
<I/σ(I)>	9.5
Completeness [%]	98.8
Redundancy	25
CC(1/2)	0.992	0.772

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291.15	0.2M Ammonium chloride, 20% w/v Polyethylene glycol 3350