9JQV

The crystal structure of SFTSV Gn and SD12 antibody complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-09-11
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.97853
Spacegroup name	P 1 21 1
Unit cell lengths	96.064, 82.342, 125.293
Unit cell angles	90.00, 104.31, 90.00

Refinement procedure

Resolution	45.920 - 2.400
R-factor	0.227
Rwork	0.225
R-free	0.25520
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.712
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.490
High resolution limit [Å]	2.400	2.400
Number of reflections	74450	66789
<I/σ(I)>	11.105
Completeness [%]	99.9
Redundancy	6
CC(1/2)	0.979	0.614

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.1 M magnesium chloride hexahydrate, 0.1 M sodium HEPES, 7.0, 15 % w/v PEG 4000