Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL10U2 |
| Synchrotron site | SSRF |
| Beamline | BL10U2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-12-24 |
| Detector | DECTRIS EIGER2 S 9M |
| Wavelength(s) | 0.97915 |
| Spacegroup name | P 1 |
| Unit cell lengths | 46.177, 54.042, 83.265 |
| Unit cell angles | 72.88, 85.96, 71.78 |
Refinement procedure
| Resolution | 48.520 - 2.290 |
| Rwork | 0.218 |
| R-free | 0.26380 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.620 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0425) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 79.550 | 2.370 |
| High resolution limit [Å] | 2.290 | 2.290 |
| Rmerge | 0.099 | |
| Number of reflections | 31319 | 3107 |
| <I/σ(I)> | 2.4 | |
| Completeness [%] | 95.2 | 95.5 |
| Redundancy | 1.7 | |
| CC(1/2) | 0.546 | 0.547 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 289.15 | 20% PEG3350 and 200 mM ammonium phosphate dibasic |
