9JQ9
Crystal structure of Plasmoredoxin from Plasmodium falciparum a disulfide oxidoreductase protein unique to Plasmodium species
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | LIQUID ANODE |
| Source details | Excillum MetalJet D2+ 160 kV |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-09-20 |
| Detector | DECTRIS PILATUS3 R 1M |
| Wavelength(s) | 1.36 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 48.551, 50.499, 78.571 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.300 - 1.900 |
| R-factor | 0.2058 |
| Rwork | 0.204 |
| R-free | 0.23670 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.935 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.300 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.062 | 0.458 |
| Rmeas | 0.064 | 0.458 |
| Rpim | 0.018 | 0.180 |
| Number of reflections | 15673 | 1517 |
| <I/σ(I)> | 26.25 | 3.77 |
| Completeness [%] | 99.4 | 98.25 |
| Redundancy | 11.6 | 6.2 |
| CC(1/2) | 0.999 | 0.957 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | ammonium acetate, ammonium sulphate, PEG-8000 |
