9JPV
A Fab Fragment of IgG
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-07-01 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 100.991, 100.991, 213.473 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.960 - 1.940 |
| R-factor | 0.2227 |
| Rwork | 0.222 |
| R-free | 0.24580 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.877 |
| Data reduction software | XDS |
| Data scaling software | XDS (1.17.1_3660) |
| Phasing software | AMoRE |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.140 | 1.980 |
| High resolution limit [Å] | 1.940 | 1.940 |
| Rmerge | 0.053 | 1.300 |
| Number of reflections | 82491 | 4480 |
| <I/σ(I)> | 32.2 | 2.2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 13.1 | 13.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1M Sodium Cacodylate pH6.5, 0.2M Calcium Acetate, 43% PEG 300, 15.8mg/mL protein |
