9JPK
Crystal structure of DhdR inducer binding domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL02U1 |
| Synchrotron site | SSRF |
| Beamline | BL02U1 |
| Temperature [K] | 177 |
| Detector technology | PIXEL |
| Collection date | 2024-09-24 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.979145 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 93.745, 64.641, 65.432 |
| Unit cell angles | 90.00, 124.21, 90.00 |
Refinement procedure
| Resolution | 31.390 - 2.280 |
| R-factor | 0.2327 |
| Rwork | 0.231 |
| R-free | 0.27060 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | AlphaFold |
| RMSD bond length | 0.026 |
| RMSD bond angle | 2.234 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 10.000 |
| High resolution limit [Å] | 2.280 | 2.280 |
| Number of reflections | 14804 | 14840 |
| <I/σ(I)> | 6.2 | |
| Completeness [%] | 98.5 | |
| Redundancy | 3 | |
| CC(1/2) | 0.895 | 0.883 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.2 M Sodium chloride, 20% w/v Polyethylene glycol 3,350, pH 7.0 |
