9JPJ
Crystal structure of DhdR in complex with DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL02U1 |
| Synchrotron site | SSRF |
| Beamline | BL02U1 |
| Temperature [K] | 177 |
| Detector technology | PIXEL |
| Collection date | 2024-09-24 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.979145 |
| Spacegroup name | P 1 |
| Unit cell lengths | 80.456, 87.044, 133.884 |
| Unit cell angles | 91.48, 102.48, 117.55 |
Refinement procedure
| Resolution | 39.260 - 3.720 |
| R-factor | 0.2526 |
| Rwork | 0.250 |
| R-free | 0.29860 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | AlphaFold |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.14-3260) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 |
| High resolution limit [Å] | 3.720 | 3.720 |
| Number of reflections | 30547 | 30388 |
| <I/σ(I)> | 12.31 | |
| Completeness [%] | 95.5 | |
| Redundancy | 4.5 | |
| CC(1/2) | 1.000 | 0.885 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 2.0 M Ammonium sulfate, 0.1 M Tris hydrochloride pH 8.5 |
