9JP6
the crystal structure of nuclease encoded by gene PAOP5_157 from Phage PaoP5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-10-14 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97923 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 58.085, 58.085, 46.044 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.080 - 2.300 |
| R-factor | 0.211 |
| Rwork | 0.206 |
| R-free | 0.25160 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.076 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21_5207) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.630 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.168 | 0.861 |
| Rmeas | 0.174 | 0.894 |
| Rpim | 0.048 | 0.241 |
| Number of reflections | 6671 | 2219 |
| <I/σ(I)> | 9.81 | 3.04 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 13.6 | 13.6 |
| CC(1/2) | 0.994 | 0.883 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289 | (w/v)25% PEG3350,0.1 M Bis-Tris pH 6.5 |
