9JOM
STRUCTURE OF ENDO-1,3-FUCANASE FUN168E FROM GH168 FAMILY
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-06-23 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97923 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 83.135, 83.135, 100.430 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 72.000 - 2.040 |
| R-factor | 0.1813 |
| Rwork | 0.177 |
| R-free | 0.23390 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.536 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 72.000 | 2.150 |
| High resolution limit [Å] | 2.040 | 2.040 |
| Rmerge | 0.230 | 0.745 |
| Rmeas | 0.246 | 0.842 |
| Rpim | 0.084 | 0.385 |
| Total number of observations | 199432 | 17782 |
| Number of reflections | 26154 | 3769 |
| <I/σ(I)> | 7.4 | 2.3 |
| Completeness [%] | 99.9 | |
| Redundancy | 7.6 | 4.7 |
| CC(1/2) | 0.989 | 0.730 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 290 | 0.1 M HEPES pH 7.5, 20% w/v Polyethylene glycol 10,000 |
