Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-10-09 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 51.637, 77.022, 111.275 |
| Unit cell angles | 90.00, 92.67, 90.00 |
Refinement procedure
| Resolution | 63.310 - 1.500 |
| R-factor | 0.17248 |
| Rwork | 0.171 |
| R-free | 0.20052 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.859 |
| Data reduction software | DIALS |
| Data scaling software | Aimless |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0425) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 63.310 | 1.530 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.057 | 0.657 |
| Rmeas | 0.070 | 0.806 |
| Rpim | 0.039 | 0.460 |
| Number of reflections | 134944 | 6568 |
| <I/σ(I)> | 11.6 | 1 |
| Completeness [%] | 97.0 | |
| Redundancy | 3.3 | |
| CC(1/2) | 0.984 | 0.528 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1 M Tris-HCl pH 8.0, 20%(w/v) PEG6000, and 0.2 M CaCl2 |
