9JNO

The complex structure of antibody CH76 bound to the hemagglutinin of influenza B virus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-05-21
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97
Spacegroup name	P 1 21 1
Unit cell lengths	78.082, 51.590, 90.540
Unit cell angles	90.00, 113.82, 90.00

Refinement procedure

Resolution	69.800 - 1.950
R-factor	0.2288
Rwork	0.226
R-free	0.27820
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.063
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	82.830	2.050
High resolution limit [Å]	1.950	1.950
Number of reflections	46623	3525
<I/σ(I)>	2.4
Completeness [%]	96.2
Redundancy	4.4
CC(1/2)	0.957	0.455

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		290	0.15M DL-Malic acid pH 7.0, 20% w/v Polyethylene glycol 3350