9JNM

Human MEX3B CBM 26-46aa peptide bound to human CNOT9

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-04-30
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.979070
Spacegroup name	P 65 2 2
Unit cell lengths	65.854, 65.854, 333.427
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.070 - 2.200
R-factor	0.1827
Rwork	0.180
R-free	0.21300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	AlphaFold
RMSD bond length	0.007
RMSD bond angle	0.847
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	PHENIX ((1.19_4092: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.630	2.270
High resolution limit [Å]	2.200	2.200
Number of reflections	22107	1880
<I/σ(I)>	11.7
Completeness [%]	97.4
Redundancy	10.5
CC(1/2)	0.991	0.912

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	sodium citrate tribasic dihydrate, PEG 500 MME