9JNL

Human MEX3B CBM 26-50aa peptide bound to human CNOT9

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-09-24
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97923
Spacegroup name	P 65 2 2
Unit cell lengths	66.321, 66.321, 334.805
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.360 - 2.400
R-factor	0.2
Rwork	0.196
R-free	0.23340
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	AlphaFold
RMSD bond length	0.009
RMSD bond angle	1.056
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	PHENIX ((1.19_4092: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.360	2.490
High resolution limit [Å]	2.400	2.400
Number of reflections	18200	1848
<I/σ(I)>	11.1
Completeness [%]	99.9
Redundancy	7.7
CC(1/2)	0.993	0.908

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	sodium citrate tribasic dihydrate, PEG 500 MME