9JNJ

Crystal structure of Human Cnot9

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-07-21
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.978530
Spacegroup name	P 1
Unit cell lengths	68.069, 68.773, 69.530
Unit cell angles	118.02, 94.29, 93.67

Refinement procedure

Resolution	42.640 - 2.000
R-factor	0.1988
Rwork	0.198
R-free	0.23690
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	AlphaFold
RMSD bond length	0.008
RMSD bond angle	0.921
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	PHENIX ((1.19_4092: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.710	2.040
High resolution limit [Å]	2.000	2.000
Number of reflections	72371	4470
<I/σ(I)>	12.5
Completeness [%]	97.1
Redundancy	3.5
CC(1/2)	0.995	0.931

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	Ammonium sulphate, PEG3350