9JM8
Crystal structure of de novo designed light-responsive heterodimer 7 (LRD-7)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | Cu FINE FOCUS |
| Temperature [K] | 181 |
| Detector technology | PIXEL |
| Collection date | 2024-08-05 |
| Detector | RIGAKU HyPix-6000HE |
| Wavelength(s) | 1 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 54.019, 38.020, 58.390 |
| Unit cell angles | 90.00, 104.15, 90.00 |
Refinement procedure
| Resolution | 28.830 - 1.400 |
| R-factor | 0.1603 |
| Rwork | 0.159 |
| R-free | 0.17770 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.087 |
| Data reduction software | CrysalisPro |
| Data scaling software | CrysalisPro |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.20.1_4487)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.830 | 1.450 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.019 | 0.132 |
| Rpim | 0.010 | 0.102 |
| Number of reflections | 22771 | 5585 |
| <I/σ(I)> | 37.08 | |
| Completeness [%] | 98.6 | |
| Redundancy | 3.7 | |
| CC(1/2) | 1.000 | 0.966 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 40% v/v Polyethylene glycol 400, 0.1 M HEPES pH 7.5 |
