9JM7
Crystal structure of de novo designed light-responsive heterodimer 2 (LRD-2)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | Cu FINE FOCUS |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-06-29 |
| Detector | RIGAKU HyPix-6000HE |
| Wavelength(s) | 1.5 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 37.701, 63.447, 123.334 |
| Unit cell angles | 90.00, 94.88, 90.00 |
Refinement procedure
| Resolution | 29.510 - 2.180 |
| R-factor | 0.2598 |
| Rwork | 0.259 |
| R-free | 0.27670 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.167 |
| Data reduction software | CrysalisPro |
| Data scaling software | CrysalisPro |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.258 |
| High resolution limit [Å] | 2.000 | 2.180 |
| Rmerge | 0.163 | 0.455 |
| Rpim | 0.057 | 0.154 |
| Number of reflections | 30407 | 2707 |
| <I/σ(I)> | 12.28 | |
| Completeness [%] | 93.2 | |
| Redundancy | 12.28 | |
| CC(1/2) | 0.976 | 0.927 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 24% (w/v) PEG 1500, 20% (v/v) Glycerol |
