9JLO
Crystal structure L-lactate dehydrogenase from Lactobacillus reuteri in its apoform
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-04-21 |
| Detector | RIGAKU HyPix-6000HE |
| Wavelength(s) | 1.54184 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 76.521, 93.124, 101.517 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 22.260 - 2.150 |
| R-factor | 0.2463 |
| Rwork | 0.244 |
| R-free | 0.28259 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6j9s |
| RMSD bond length | 0.015 |
| RMSD bond angle | 2.132 |
| Data reduction software | CrysalisPro |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 22.260 | 2.220 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.273 | 0.814 |
| Rmeas | 0.293 | 0.889 |
| Rpim | 0.105 | 0.351 |
| Total number of observations | 20819 | |
| Number of reflections | 40098 | 3403 |
| <I/σ(I)> | 5.4 | 1.8 |
| Completeness [%] | 99.8 | |
| Redundancy | 7.7 | 6.1 |
| CC(1/2) | 0.981 | 0.805 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 288 | 1% w/v Tryptone, 0.001 M Sodium azide, 0.05 M HEPES sodium pH 7.0, 12% w/v Polyethylene glycol 3350 |
