9JLK

NADP-dependent oxidoreductase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-07-10
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97918
Spacegroup name	P 41 21 2
Unit cell lengths	89.708, 89.708, 203.747
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	18.550 - 2.330
R-factor	0.1936
Rwork	0.191
R-free	0.24030
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	0.986
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX ((???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	89.710	2.390
High resolution limit [Å]	2.330	2.330
Number of reflections	36508	2647
<I/σ(I)>	19.3
Completeness [%]	100.0
Redundancy	26
CC(1/2)	0.999	0.969

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	9	277.15	PEG3350,sodium chloride,bis-tris hydrochloride