9JL0

Crystal structure of feline CD8aa homodimer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-04-09
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.979
Spacegroup name	P 41 21 2
Unit cell lengths	132.750, 132.750, 44.660
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.330 - 2.480
R-factor	0.2094
Rwork	0.205
R-free	0.25020
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.973
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.17.1_3660))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.330	2.540
High resolution limit [Å]	2.480	2.480
Rmerge	0.142
Rmeas	0.145
Rpim	0.027
Number of reflections	14683	30533
<I/σ(I)>	24.35	8.1
Completeness [%]	100.0	100
Redundancy	20
CC(1/2)	0.999	0.973

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	277	0.2 M Magnesium sulfate heptahydrate, 20% w/v polyethylene glycol 3350, pH 6.0