9JKO

Crystal structure of the RNA- binding domain of human ZAR1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-03-31
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.97938
Spacegroup name	P 21 21 21
Unit cell lengths	47.610, 58.450, 81.500
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.510 - 1.630
R-factor	0.19
Rwork	0.188
R-free	0.21620
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.140
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	27.510	1.690
High resolution limit [Å]	1.630	1.630
Rmerge		1.780
Number of reflections	29055	1998
<I/σ(I)>	10.3
Completeness [%]	100.0
Redundancy	12.6
CC(1/2)	0.984	0.714

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.1 M Succinic acid pH 7.0, 20% w/v Polyethylene glycol 3350