9JKL

LYSYL-TRNA SYNTHETASE(LysRS) COMPLEXED WITH LYSINE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ELETTRA BEAMLINE 11.2C
Synchrotron site	ELETTRA
Beamline	11.2C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-02-02
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0
Spacegroup name	P 41 21 2
Unit cell lengths	105.015, 105.015, 241.350
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	79.220 - 2.300
Rwork	0.204
R-free	0.22800
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	79.220	2.360
High resolution limit [Å]	2.300	2.300
Rmerge	0.138	1.524
Rmeas	0.141	1.556
Rpim	0.028	0.309
Number of reflections	60972	4444
<I/σ(I)>	16.9	2.6
Completeness [%]	100.0	100
Redundancy	24.8	25.2
CC(1/2)	0.999	0.959

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH		296	0.1M Tris HCl pH 8.5, 8% W/V PEG 8000, 5mM Lysine