9JKC

Crystal structure of Aspergillus fumigatus polymycovirus 1 ploymerase (residues 85-763) in its apo state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-06-11
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97917
Spacegroup name	C 1 2 1
Unit cell lengths	257.961, 182.916, 118.681
Unit cell angles	90.00, 103.79, 90.00

Refinement procedure

Resolution	29.880 - 3.400
R-factor	0.1601
Rwork	0.158
R-free	0.20560
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Cyro-EM structure of AfuPmV-1 N85
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.19)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	40.000	40.000	3.520
High resolution limit [Å]	3.400	7.310	3.400
Rmerge	0.141	0.039	0.492
Rmeas	0.168	0.046	0.592
Rpim	0.090	0.025	0.324
Total number of observations	245809
Number of reflections	72936	7333	7273
<I/σ(I)>	8
Completeness [%]	99.8	98.7	99.8
Redundancy	3.4	3.2	3.2
CC(1/2)		0.997	0.750

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	8.5	289	Tris pH 8.5, SOKALAN CP 42