9JJX

Truncated RNF112, GDP-bound form 2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-11-20
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.97918
Spacegroup name	P 1
Unit cell lengths	39.390, 48.387, 124.029
Unit cell angles	96.44, 96.25, 90.42

Refinement procedure

Resolution	31.450 - 2.730
R-factor	0.2462
Rwork	0.244
R-free	0.29350
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.513
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX ((1.19_4092: 000))
Refinement software	PHENIX ((1.19_4092: 000))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	122.490	2.880
High resolution limit [Å]	2.730	2.730
Number of reflections	23446	3419
<I/σ(I)>	5.7	2.6
Completeness [%]	98.0
Redundancy	3.2
CC(1/2)	0.977	0.860

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291.15	PEG 3350, Tacsimate pH 6.0