9JJW

Truncated RNF112, GDP-bound form 1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-11-22
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97853
Spacegroup name	C 2 2 21
Unit cell lengths	91.461, 147.377, 71.111
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.280 - 2.090
R-factor	0.1865
Rwork	0.184
R-free	0.22100
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.043
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX ((1.19_4092: 000))
Refinement software	PHENIX ((1.19_4092: 000))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	77.710	2.200
High resolution limit [Å]	2.090	2.090
Number of reflections	28846	4143
<I/σ(I)>	16.5	2.6
Completeness [%]	99.9	99.9
Redundancy	5.8	5.4
CC(1/2)	0.999	0.819

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291.15	Tacsimate pH 7.0,