9JJV

Truncated RNF112, transition-like state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-08-21
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.97918
Spacegroup name	C 2 2 21
Unit cell lengths	91.567, 148.057, 71.315
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.010 - 1.880
R-factor	0.1882
Rwork	0.186
R-free	0.22350
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.088
Data reduction software	DIALS
Data scaling software	DIALS
Phasing software	PHENIX ((1.19_4092: 000))
Refinement software	PHENIX ((1.19_4092: 000))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	77.880	1.930
High resolution limit [Å]	1.880	1.880
Number of reflections	39475	2689
<I/σ(I)>	14.5	2
Completeness [%]	99.2	92.8
Redundancy	11.5	6.1
CC(1/2)	0.999	0.609

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291.15	PEG 3350, Tacsimate pH 7.0,