9JIU
Ferritin mutant R63MeHis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL15A1 |
| Synchrotron site | NSRRC |
| Beamline | BL15A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2024-05-01 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 42 21 2 |
| Unit cell lengths | 219.169, 219.169, 147.906 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.890 - 2.280 |
| R-factor | 0.2392 |
| Rwork | 0.239 |
| R-free | 0.27180 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.584 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.360 |
| High resolution limit [Å] | 2.280 | 2.280 |
| Rmerge | 0.200 | 0.783 |
| Rmeas | 0.223 | 0.843 |
| Rpim | 0.080 | 0.305 |
| Number of reflections | 159010 | 14962 |
| <I/σ(I)> | 8.4 | 2 |
| Completeness [%] | 97.5 | 93.1 |
| Redundancy | 7.3 | 7.2 |
| CC(1/2) | 0.949 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 0.1M Bicine pH 8.5, 20%(v/v) PEG 300 |
