Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2022-09-10 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9791 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 77.540, 84.447, 63.838 |
| Unit cell angles | 90.00, 100.96, 90.00 |
Refinement procedure
| Resolution | 39.313 - 1.996 |
| R-factor | 0.2195 |
| Rwork | 0.218 |
| R-free | 0.24410 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.974 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.313 | 2.120 |
| High resolution limit [Å] | 1.996 | 2.000 |
| Rmerge | 0.082 | |
| Number of reflections | 26511 | 2710 |
| <I/σ(I)> | 9.62 | |
| Completeness [%] | 95.8 | |
| Redundancy | 3.4 | |
| CC(1/2) | 0.997 | 0.922 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1M Tris-HCl pH 8.5, 0.1M Bicine pH 8.5, 0.03 M NaF, 0.03 M NaBr, 0.03 M NaI, 25%(w/v) MPD, 25%(w/v) PEG 1000, 25%(w/v) PEG 3350 |
