9JHT
Jumbo phage Killer B
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-04-17 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97852 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 83.260, 98.850, 199.430 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.520 - 2.440 |
| R-factor | 0.2345 |
| Rwork | 0.233 |
| R-free | 0.26830 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.500 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.330 | 2.500 |
| High resolution limit [Å] | 2.440 | 2.440 |
| Number of reflections | 31049 | 2268 |
| <I/σ(I)> | 17.9 | |
| Completeness [%] | 99.9 | |
| Redundancy | 13.4 | |
| CC(1/2) | 0.999 | 0.762 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 10% (w/v) PEG 3000, 100 mM Sodium cacodylate/ Hydrochloric acid pH 6.5, 200 mM Magnesium chloride |
